| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076867 |
| Chromosome: | chromosome 6 |
| Location: | 1805592 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263357 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGTGGTCAAAGTACACGACGAGGTATCGAGGTCGGCTGGCACCCCG |
| Internal bar code: | AACGGTCAGAACCCAACCCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1940 |
| LEAP-Seq percent confirming: | 84.8485 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTCCTTCAATGCGGCTGC |
| Suggested primer 2: | AACTTACTCCTTGGTGCCGG |