Insertion junction: CLIP2.076868_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.076868
Chromosome:chromosome 1
Location:5218044
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre01.g036451 SMM2 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCAAGCCTCCCTTTCCTTCGCCCCACCTTCCGACTTCGCACCTCGCG
Internal bar code:ACTATCGTTTTATTCTTGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1398
LEAP-Seq percent confirming:42.8571
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGAATCCCTCACACACCC
Suggested primer 2:AACGCATCGTAGAGCTCCTG