| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.076879 |
| Chromosome: | chromosome 5 |
| Location: | 181348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242700 | SUB9 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGTCCTCCTCCACGTATCCGCACACCCTGGCAGCCATCCTCCATAGA |
| Internal bar code: | GCGTCGAGTACTTACGTGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAAGTACCCTCAGTGTGT |
| Suggested primer 2: | CTTGGAGGGCCCTTACTTGG |