| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076909 |
| Chromosome: | chromosome 9 |
| Location: | 5764352 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410900 | NAR2,NIT8 | nitrite transporter accessory protein; (1 of 1) PF16974 - High-affinity nitrate transporter accessory (NAR2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCAGGCTACGCTAGGCAGGCCTGTGGCTGAAGGGGCACCGGGCTCAG |
| Internal bar code: | ACTACTAGACGATTACGCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCTGAAGCCTGGCAGAC |
| Suggested primer 2: | GTGCGTGTGGTCTGTGACTA |