Insertion junction: CLIP2.076909_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.076909
Chromosome:chromosome 9
Location:5764354
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g410900 NAR2,NIT8 nitrite transporter accessory protein; (1 of 1) PF16974 - High-affinity nitrate transporter accessory (NAR2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTTCATTTGTCGATGCATGGGTTCACAATGCACCTCGGTATTGAGTG
Internal bar code:TGCAGCGAGTACGTCGACCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:308
LEAP-Seq percent confirming:3.22581
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCGTGTGGTCTGTGACTA
Suggested primer 2:ACATCTGAAGCCTGGCAGAC