| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.076915 |
| Chromosome: | chromosome 2 |
| Location: | 540600 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076700 | outside_mRNA | ||
| Cre02.g076750 | CIA30,NUOAF1,NUOFAF1 | NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 1; (1 of 1) K18159 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 (NDUFAF1, CIA30) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGCAATGAGGCTCTTCCCTTCCCTTGGATTGAAGCGAACCGTTCTC |
| Internal bar code: | ATTTGCAAGTCCCCAGGACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCCAGACCTCCTTTCCA |
| Suggested primer 2: | ACGTGTCACAAACGCATTGG |