| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.076919 |
| Chromosome: | chromosome 2 |
| Location: | 6350444 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387800 | FER1 | Pre-apoferritin; (1 of 2) K00522 - ferritin heavy chain (FTH1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCACCAGTGACTTCTGGTACTTGCATTACATCATACATCTAGAATTT |
| Internal bar code: | TAGTAGTAGGTATTCCTCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCACACAGGCAAAGCAGG |
| Suggested primer 2: | ATGTGCGTTTTTGGGACACG |