| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076923 |
| Chromosome: | chromosome 12 |
| Location: | 2842603 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503300 | (1 of 1) 4.1.1.21 - Phosphoribosylaminoimidazole carboxylase / AIR carboxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTGATCCGCCCTTCCCCGCCCCGCACGTCACACCGCACGTCACGCC |
| Internal bar code: | AACCCATCGCTAGCCAATAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1503 |
| LEAP-Seq percent confirming: | 3.125 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCCTCCACTCGTCTCC |
| Suggested primer 2: | GGCGTGCATGTTTCATGTGT |