Insertion junction: CLIP2.076927_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.076927
Chromosome:chromosome 2
Location:2741310
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTCGTTTGCCATGACGAGGACGACATGATGATAAGCCATCCGTGTGTG
Internal bar code:GAGAGTCAGTGTTAGATCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1673
LEAP-Seq percent confirming:40.9091
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCAATGCAACACGGTGA
Suggested primer 2:GAACGAGTACCACAGCCACA