| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.076929 |
| Chromosome: | chromosome 7 |
| Location: | 4741076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345500 | (1 of 2) PF13875 - Domain of unknown function (DUF4202) (DUF4202) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTCCCCACCCACACCGCCCGCTCCTGGTCAATGCACTCGGTCGCTGC |
| Internal bar code: | TCTGCAAGACAGGTTTTACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1814 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTTCCTGCAGCTTCTA |
| Suggested primer 2: | ACGCAAATTGGCAATGCTGT |