| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076930 |
| Chromosome: | chromosome 6 |
| Location: | 2127778 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800630 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAGCAGCACTGACAGCGCTTCACATGCGATTGCACCACAGATTCGCG |
| Internal bar code: | GTGTTATGAGTCCCTCATGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 1.96078 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACAAGCATTCCGGCA |
| Suggested primer 2: | CTGGTGAAGGTGGTGGAGTC |