| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076946 |
| Chromosome: | chromosome 6 |
| Location: | 6427927 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293582 | TSL2 | Putative leucyl-tRNA synthetase; (1 of 1) PTHR11946//PTHR11946:SF51 - ISOLEUCYL, LEUCYL, TYROSYL, VALYL AND METHIONYL-TRNA SYNTHETASES // LEUCINE--TRNA LIGASE, CYTOPLASMIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACACGCACAAGCGCGTCGCGTCGTTGGGCGGGCTTTTGTTTCGTCT |
| Internal bar code: | CGATCTACCTCTTCAGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCTCTCCCACTCCCCAC |
| Suggested primer 2: | TATATGTGAGCGCGCGAAGT |