| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.076952 |
| Chromosome: | chromosome 2 |
| Location: | 2412568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091200 | (1 of 1) 1.13.11.5 - Homogentisate 1,2-dioxygenase / Homogentisicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGACTACTGGAGCAACATTTATATCGACACAACTATGATTAACAAAC |
| Internal bar code: | GGTTGCTTTCGGTGGAGGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1274 |
| LEAP-Seq percent confirming: | 17.2414 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGGGTGGGCATTTCATA |
| Suggested primer 2: | CCATCGGCCATCAGCAGTAA |