| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076954 |
| Chromosome: | chromosome 6 |
| Location: | 2135189 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800632 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAGTTAGTGCAGTCTATTGATATTGACGTGCTTAAAAAAACGCAACA |
| Internal bar code: | GGGAACATTGTCACCCCCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3369 |
| LEAP-Seq percent confirming: | 1.81818 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTACACAGGCCTCGGATAC |
| Suggested primer 2: | GCATGCTGATAAGTGGTGCG |