| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076962 |
| Chromosome: | chromosome 2 |
| Location: | 5994456 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118500 | COPB2 | (1 of 1) K17302 - coatomer, subunit beta' (COPB2, SEC27); Beta' subunit of COP-I complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGATCTACTCTACAAATGGTTTCCGATACTTGGGAATGCATGGATAG |
| Internal bar code: | AGTGCCGTAAGTGAATTCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2389 |
| LEAP-Seq percent confirming: | 29.4118 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAGAAGTGAGTGCTGAG |
| Suggested primer 2: | TCCATGGTTCGGTTGGACTG |