Insertion junction: CLIP2.076964_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.076964
Chromosome:chromosome 1
Location:4304705
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g029200 DIV30,ESP1 Separase, cell cycle protease; (1 of 1) 3.4.22.49 - Separase / Separin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTCCTCTTCTACCGGTCTACAGCTGTACATCACTGATGCAGCGGCAG
Internal bar code:GTACCCCTCCTTTAATTTATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2881
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TATGCTCTCAAACGCACGGT
Suggested primer 2:CCCTTCTTGTGGCTTTGTGC