| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.076978 |
| Chromosome: | chromosome 12 |
| Location: | 5139918 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526326 | (1 of 3) K01764 - cytochrome c heme-lyase (E4.4.1.17) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCACATACAGCCGGCGCCGCCGCCGGACCCAGCCCCCGGCCGCGGGCG |
| Internal bar code: | ATAGTTTAGGTTTTGGGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3748 |
| LEAP-Seq percent confirming: | 1.16279 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCCCAATCAAGCAGGT |
| Suggested primer 2: | TACCTTCTTTGCCTTCGCGT |