| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.076989 |
| Chromosome: | chromosome 5 |
| Location: | 3299546 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232450 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACGATTGGCCTCGCGTGTGATAGTCCTCAGATTTCCAACGCTTTAC |
| Internal bar code: | GTACTAAGTAAACTGAAGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 421 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTCTAGACACCGAGAG |
| Suggested primer 2: | GGCTCACGCAACAACACAAT |