| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.076991 |
| Chromosome: | chromosome 2 |
| Location: | 6680625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387134 | (1 of 3) PF04379 - Protein of unknown function (DUF525) (DUF525) | 5'UTR | |
| Cre09.g387171 | RHM1,DIV109,EZY22 | nucleotide-rhamnose synthase/epimerase-reductase, dTDP-4-dehydrorhamnose reductase / dTDP-6-deoxy-L-mannose dehydrogenase; (1 of 1) 1.1.1.133 - dTDP-4-dehydrorhamnose reductase / dTDP-6-deoxy-L-mannose dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAACTACTCTGCTTTGACTGGCATGAACCATGGACCGGATACACGACT |
| Internal bar code: | CTAATTGGACTGCATATTCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1176 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACGAGCATTAGAGCGTG |
| Suggested primer 2: | AAGGAGCCTACGAGGTGCTA |