| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077003 |
| Chromosome: | chromosome 14 |
| Location: | 1373694 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616826 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCGTCCCAAACATGTCTCCCCTATAGCACCCAAACGATCGTTACGAT |
| Internal bar code: | AATGACAGAGCGGGGGTTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3175 |
| LEAP-Seq percent confirming: | 3.52941 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCTGGCGCTAGAGATGG |
| Suggested primer 2: | TCAGTCGTCTAGGTCCAGCA |