| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077010 |
| Chromosome: | chromosome 10 |
| Location: | 3152643 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441700 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGACAGGAAGCGCCAAGCACGAATGTAGGCAGCAGGCACCTTCATAGA |
| Internal bar code: | TTTGTGGGCAGAGCTTTGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2458 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGGGAATGAGGAGGAGG |
| Suggested primer 2: | CGGCCGACATAGTTTGCATG |