| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077053 |
| Chromosome: | chromosome 7 |
| Location: | 2469373 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329277 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATAGCAACATTTATTCCTTCAATAATTGAGGGCACTCGAGTTACTGT |
| Internal bar code: | ATGTAGGATCCTTAACCGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1605 |
| LEAP-Seq percent confirming: | 91.1111 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTTGCGTGAGAGAGGTC |
| Suggested primer 2: | CGTGATTGGCGATACCGAGA |