Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.077056 |
Chromosome: | chromosome 3 |
Location: | 5607742 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g185800 | (1 of 14) PF00892 - EamA-like transporter family (EamA) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACTATTTCAACTAGCGCGGAGCGAGATTGCCATCACTGCCTCACTGGC |
Internal bar code: | ATTATATCATTTATAGGGTCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2235 |
LEAP-Seq percent confirming: | 95.7747 |
LEAP-Seq n confirming: | 68 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCAGCTGGAAAGGAAGGGG |
Suggested primer 2: | AGCACGACTGCCATGTGTTA |