| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077056 |
| Chromosome: | chromosome 3 |
| Location: | 5613693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185800 | (1 of 14) PF00892 - EamA-like transporter family (EamA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCCCGCACCTGAGTGACGAGGACGCGGAGGACCTGGTGGGGCAGCTG |
| Internal bar code: | CGCGCAGGGCGGAGAACAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2171 |
| LEAP-Seq percent confirming: | 96.3855 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGACTGCCATGTGTTA |
| Suggested primer 2: | CTCAGCTGGAAAGGAAGGGG |