Insertion junction: CLIP2.077058_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.077058
Chromosome:chromosome 10
Location:6551614
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g465550 CLPD1,CLPD (1 of 2) 3.6.4.10 - Non-chaperonin molecular chaperone ATPase / Molecular chaperone Hsc70 ATPase; ClpD chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACACCCCACATAAGGACTTAGTTGCTTTCTGCCGTCCAGGCCCCCTC
Internal bar code:GGTCTGAACTTTTGAGCCCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:145
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGCGACCTTAGGAGTGCG
Suggested primer 2:AGGTGTGTGTGTGTGTGTGT