| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.077061 |
| Chromosome: | chromosome 17 |
| Location: | 2225709 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTTGAGACTCAATGTTGCCGTGGGTGAGCGCAGTATGGTAGTACGGC |
| Internal bar code: | TCCGCCTTGTTTTGCGCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTGAGTCTTTGCCTCT |
| Suggested primer 2: | CGTTGTACAACCAGACCGGA |