| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077080 |
| Chromosome: | chromosome 1 |
| Location: | 7848011 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055408 | ACS2 | (1 of 3) K01895 - acetyl-CoA synthetase (ACSS, acs); Acetyl-CoA synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGTGGAGGCGGAGCACCCGCTGTTCCTGCTGTACACCTCGGGGTCCA |
| Internal bar code: | GACCCTAGCACGTATTAATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTCAGGAGGAGAAGGGA |
| Suggested primer 2: | AGAAGATTGCGGACAAGGCA |