| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.077105 |
| Chromosome: | chromosome 10 |
| Location: | 5988665 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461450 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCATCGACTCACTCACCGCGGGCCTCACCTGGCTGTATCTTGGAGT |
| Internal bar code: | ACGTTGTTTAGGCACACAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2209 |
| LEAP-Seq percent confirming: | 48.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCCGCCATCTACTGAGC |
| Suggested primer 2: | GGTACAGGTGGTGGTGATGG |