| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077121 |
| Chromosome: | chromosome 7 |
| Location: | 4771757 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345850 | MRPL35,bL35m | Mitochondrial ribosomal protein L35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACGGGATGGGCCTCATTCCAGAATCCTCCAAAATCAACAAGCTAGCTT |
| Internal bar code: | TCTAGCTCCGAGAGGTTAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATGGGGATGAGGAGGTT |
| Suggested primer 2: | ACGAGTCAGCACTTGCATGA |