| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077125 |
| Chromosome: | chromosome 1 |
| Location: | 5476591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038750 | MOT7 | (1 of 1) IPR007024 - BLUF domain; motile cilia related | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCAGGAGGAGTTGTCAGTGCGCCACAGCGGCACAGGTTAAGCACAC |
| Internal bar code: | TATTGCACGGCATTTTACTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4413 |
| LEAP-Seq percent confirming: | 91.8367 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGAGTGGACAGGGTTAA |
| Suggested primer 2: | CCATGGTGTGTTAGGGCCAT |