| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.077126 |
| Chromosome: | plastome |
| Location: | 10378 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802266 | 2716978,psbK,ChreCp004 | (1 of 1) K02712 - photosystem II PsbK protein (psbK); photosystem II K protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAAAAACATACTCTTTACGGGTACGCGAATGTTAGGTAAATTTTCAC |
| Internal bar code: | GAAAACTGCCCGGAGTCTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 10.5263 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCTTTCAGGCAACCCAA |
| Suggested primer 2: | CATAGGGTCGCAGGTTCGAA |