| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077129 |
| Chromosome: | chromosome 11 |
| Location: | 3099288 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475150 | Similar to PKD2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGCTCGCCTAGCCCTGGTAGTAGCTTTCGTCGGTGTGAATTGCGGT |
| Internal bar code: | CGACGACAGGGTTTGTATCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1256 |
| LEAP-Seq percent confirming: | 32.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCCCATGTCCATGACAC |
| Suggested primer 2: | CCAGGGAACTTGCTGCAGTA |