Insertion junction: CLIP2.077132_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.077132
Chromosome:chromosome 2
Location:1416970
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre02.g083400 (1 of 1) PF07000 - Protein of unknown function (DUF1308) (DUF1308) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCCGCACCCACCCACCTATCTTGCATCCGGTCGGCGGCCCTCCCTCG
Internal bar code:CGGGGTATAGGTTTAGTATAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:116
LEAP-Seq percent confirming:24.3902
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAATGTTTGCGATGCCG
Suggested primer 2:CTCTGTTGCTGCTGCTGTTG