| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077135 |
| Chromosome: | chromosome 17 |
| Location: | 1349825 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705836 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; Chlamydomonas-specific family W protein | 5'UTR | |
| Cre17.g705843 | Chlamydomonas-specific family W protein; (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAAAGACGTTGCAAGTAGCGGTCTTCCTCGGCAGGCTCATCAGCAAGA |
| Internal bar code: | GCGTGTGCACGAAGACGCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3987 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCCGTTGCGTCGTCTTA |
| Suggested primer 2: | TGATCGATGCCGTAAGCCTC |