Insertion junction: CLIP2.077143_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.077143
Chromosome:chromosome 8
Location:2544854
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACACACATACGCATGCATACACACGCCACTCGCCACCG
Internal bar code:ATGACGCTTGGCGGAAACGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:149
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTGCTCCTCCAGTTTGGG
Suggested primer 2:GCCCTGGATGATATCAGGCC