| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077148 |
| Chromosome: | chromosome 2 |
| Location: | 547273 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076800 | ERG4 | Delta14-sterol reductase, mitochondrial; (1 of 1) 1.3.1.70 - Delta(14)-sterol reductase / Sterol C14-reductase | 5'UTR |
| Cre02.g076850 | (1 of 1) PTHR19854:SF1 - GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATTCACGAAGGTCACTATATACAGACTGCAGCCGCAGTCAACTTGCC |
| Internal bar code: | AGCGGTACGTATCGAACAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5764 |
| LEAP-Seq percent confirming: | 99.3056 |
| LEAP-Seq n confirming: | 143 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTACGACCTCATCACGG |
| Suggested primer 2: | TGACAGTCTGTGGAAGCGAC |