| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077157 |
| Chromosome: | chromosome 12 |
| Location: | 2231983 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508700 | ELG2 | Exostosin-like glycosyltransferase; (1 of 1) K02367 - glucuronyl/N-acetylglucosaminyl transferase EXT2 (EXT2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTAAGTTCGGGTCATTTGTGGCAGGCCAGTCGCTCAAAAGAGTAGTAA |
| Internal bar code: | TAGTTTCAGCCGCTAGAAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4244 |
| LEAP-Seq percent confirming: | 91.0256 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTATGGAGAGCATGCGT |
| Suggested primer 2: | CAGGTACAGGCAGTGAGTGG |