Insertion junction: CLIP2.077166_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.077166
Chromosome:chromosome 6
Location:594349
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g253200 PKY1 Protein tyrosine kinase; (1 of 1) PTHR23257:SF534 - SERINE/THREONINE-PROTEIN KINASE ROCO7-RELATED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCTGCGGCGCTGTTGTACCGCGTGGCTCTGGACATCGCCAACGGCATG
Internal bar code:ATCAGCATTTTACACATACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:974
LEAP-Seq percent confirming:13.0435
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CAACACAGGAACCGTCAGGA
Suggested primer 2:GCGTCACCGGCATTGTATTC