| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077177 |
| Chromosome: | chromosome 10 |
| Location: | 4651322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451800 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACTCGGCTGGGCTCTGCTTGGGCTGTGTAAAAGTCTTGCGCCGCTGC |
| Internal bar code: | GAAGGAATTACTCGTGGTGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1322 |
| LEAP-Seq percent confirming: | 56.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACAGCCACGGCATAGCAA |
| Suggested primer 2: | GTAACGCACCCAGACTCACA |