| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077208 |
| Chromosome: | chromosome 8 |
| Location: | 3615907 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379700 | PHM1 | (1 of 1) 5.4.2.3 - Phosphoacetylglucosamine mutase / N-acetylglucosamine-phosphate mutase; Phosphoacetylglucosamine/phosphohexo mutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGCGTGGGTGGCTTGTGGGCGTGGGGTGGTGTGGGACGTGGCACAG |
| Internal bar code: | TTCTATGGTCTTATCGATTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAGCAGCAGTTACAGCA |
| Suggested primer 2: | CCCACTCCACGTTACGAACA |