| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.077213 |
| Chromosome: | chromosome 12 |
| Location: | 5964527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533950 | COT1 | Cobalt transport protein; (1 of 1) PTHR33514//PTHR33514:SF3 - FAMILY NOT NAMED // PROTEIN ABCI12, CHLOROPLASTIC | 3'UTR |
| Cre12.g534000 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGGGTTGGGGTGTTTGTGCATGCGCGTATGTTGGAGGTGGCAATCG |
| Internal bar code: | TGACTGGCTCTTTCAACATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2607 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGTGAGCCAAGTCGGTG |
| Suggested primer 2: | TCCTGGATCAGTTGCGGAAC |