Insertion junction: CLIP2.077229_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.077229
Chromosome:chromosome 3
Location:5033042
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g800342 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACGCACACGCACACGCACGCACACATATACACACGCACACACACA
Internal bar code:TCGCGGTGGAGACCTGTGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:383
LEAP-Seq percent confirming:6.66667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GACTTTTGCACTTGGCCTGG
Suggested primer 2:ATTCACTTTCCCCAGGCGAG