| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077236 |
| Chromosome: | chromosome 5 |
| Location: | 494108 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244100 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATAAAAATTGTGGAGGTGCTACTGCGTCAGCAAAGACATGTTGCGTG |
| Internal bar code: | ACGGTTGTGGGTCGTCTTAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 31.25 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCGCAGGTCAAAGCAAA |
| Suggested primer 2: | TGCCACCACCATCATTCTCC |