| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077239 |
| Chromosome: | chromosome 3 |
| Location: | 4090421 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172250 | (1 of 1) PTHR11649:SF5 - GTP-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACACACACGCCCCCAACCCCACAGAGATGCCGCAGACGGTGTACGA |
| Internal bar code: | TGACACTCGAATTGTATTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 59.2593 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCATCGTCCGAGTCCG |
| Suggested primer 2: | CAAATCCTGCCTTGGCACAC |