| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077249 |
| Chromosome: | chromosome 12 |
| Location: | 2191088 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509200 | (1 of 1) PF00722//PF03935 - Glycosyl hydrolases family 16 (Glyco_hydro_16) // Beta-glucan synthesis-associated protein (SKN1) (SKN1); weakly Similar to Beta-Glucan Synthesis-Associated | 3'UTR | |
| Cre12.g801375 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGTCTCACCATGTCACACTACCAGTCTCACACGCGCAGTATCGCAC |
| Internal bar code: | GCGTCGCAAAGACAGGAAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 528 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTATTAAGCGGGCGTGGC |
| Suggested primer 2: | TGGAGCTGCTGAACAAGGAG |