Insertion junction: CLIP2.077251_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.077251
Chromosome:chromosome 3
Location:1897303
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre03.g154850 COQ4 Ubiquinone biosynthesis protein; (1 of 1) K18586 - ubiquinone biosynthesis protein COQ4 (COQ4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGTGCTCTTCGACATAAGTTTGTAACACACAACACACACGCACACACC
Internal bar code:GCCGTCCGAGTCCTTGCTCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:462
LEAP-Seq percent confirming:38.2353
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTGTGTTGTTCTGTCACC
Suggested primer 2:GCCATCGCCCTGTCATGATA