| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.077256 |
| Chromosome: | chromosome 3 |
| Location: | 671119 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145727 | (1 of 1) PTHR11946:SF53 - TYROSINE-TRNA LIGASE | outside_mRNA | |
| Cre03.g145747 | SHK7,SHKH1 | (1 of 1) 4.2.3.5 - Chorismate synthase / 5-enolpyruvylshikimate-3-phosphate phospholyase; Chorismate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACAGATCTTGCGTAGCCGCCCTGCCCCAGCCAACATGCCTCAAAATTT |
| Internal bar code: | AGCTACTTCCAACGGAAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1372 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTTGTATGGAGCGGGGA |
| Suggested primer 2: | GCTGATGCTGTTTAGGGGGT |