| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.077259 |
| Chromosome: | chromosome 1 |
| Location: | 5040291 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034950 | (1 of 1) K06184 - ATP-binding cassette, subfamily F, member 1 (ABCF1); Soluble ABC-F domain containing protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGGATCGAGTTTGGCACGCCCATCCCGGCCGTGGAACCTTTCCCTGG |
| Internal bar code: | GTCATTTTCGCGATTTGTATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1153 |
| LEAP-Seq percent confirming: | 88.5714 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGTTCCCAGCCTTCGCAG |
| Suggested primer 2: | GTCCCCCTTCTCTTCCTTGC |