Insertion junction: CLIP2.077262_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.077262
Chromosome:chromosome 2
Location:4940292
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g110150 (1 of 1) PF00651//PF03110//PF07707 - BTB/POZ domain (BTB) // SBP domain (SBP) // BTB And C-terminal Kelch (BACK) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCTAGATTGCTCCACGGCGCCATGCTGGCATGGTCGCTGTGGCAACGC
Internal bar code:CAAGAATAGGTTTCGGCGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4407
LEAP-Seq percent confirming:60.5505
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:43
LEAP-Seq n unique pos:109

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCGTTTTGCGCTTATGAT
Suggested primer 2:CCAAACTTGTTCAGGCCGTG