| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.077270 |
| Chromosome: | chromosome 10 |
| Location: | 3794947 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445600 | (1 of 1) K14569 - ribosome biogenesis protein BMS1 (BMS1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATGTTTCAGTTCTCTTATATGACGTCCACTCGCTGTATTAATATGAG |
| Internal bar code: | GCCTTCGCCATTTCAGTACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2592 |
| LEAP-Seq percent confirming: | 43.6364 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCTTTTGCGGGTCACT |
| Suggested primer 2: | ACTCTCCCACCCATAGACCC |